Development of an optical fiber interferometer for detection of surface flaws in aluminum

The main objective was to demonstrate the potential of using an optical fiber interferometer (OFI) to detect surface flaws in aluminum samples. Standard ultrasonic excitation was used to generate Rayleigh surface waves. After the waves interacted with a defect, the modified responses were detected using the OFI and the results were analyzed for time-of-flight and frequency content to predict the size and location of the flaws

Endoscopic measurements using a panoramic annular lens

The objective of this project was to design, build, demonstrate, and deliver a prototype system for making measurements within cavities. The system was to utilize structured lighting as the means for making measurements and was to rely on a stationary probe, equipped with a unique panoramic annular lens, to capture a cylindrical view of the illuminated cavity. Panoramic images, acquired with a digitizing camera and stored in a desk top computer, were to be linearized and analyzed by mouse-driven interactive software

Endoscopic inspection using a panoramic annular lens

The objective of this one year study was to design, build, and demonstrate a prototype system for cavity inspection. A cylindrical view of the cavity interior was captured in real time through a compound lens system consisting of a unique panoramic annular lens and a collector lens. Images, acquired with a digitizing camera and stored in a desktop computer, were manipulated using image processing software to aid in visual inspection and qualitative analysis. A detailed description of the lens and its applications is given

Predicting Trade Expansion under FTAs and Multilateral Agreements

This paper examines the historical record of eight recent free trade agreements (FTAs). It also investigates the predictive power of two popular quantitative world trade models—the single-equation gravity model and the multiequation comput-able general equilibrium (CGE) model—as applied to three major trade liberalization agreements adopted during the 1990s: Mercosur, NAFTA, and the Uruguay Round Agreement, using the Rose gravity model and the GTAP general equilibrium model. Both models are found accurate in some instances, but intervening influences in the wake of trade liberalization episodes confound the challenge of drawing a strong conclusion in favor of one modeling approach over the other. Between the “naïve” gravity model and “naïve” CGE model predictions, we find that the former tends to overpredict intrabloc trade expansion (especially over horizons of five years and less) while the latter tends to underpredict. CGE models remain favored for ex post analysis of welfare impacts and the direct and indirect linkages between policy reforms and the numerous other economic variables of concern to policymakers and the public at large.gravity models, CGE models, regional trading arrangements

How rapid is aphid-induced signal transfer between plants via common mycelial networks?

Peer reviewedPublisher PD

Increasing phosphorus supply is not the mechanism by which arbuscular mycorrhiza increase attractiveness of bean (Vicia faba) to aphids

Peer reviewedPublisher PD

Stool consistency as a major confounding factor affecting microbiota composition : an ignored variable?

Author Posting. © The Author(s), 2015. This is the author's version of the work. It is posted here by permission of BMJ Publishing Group for personal use, not for redistribution. The definitive version was published in Gut 65 (2016): 1-2, doi:10.1136/gutjnl-2015-310043.2016-07-1

Membership and behavior of ultra-low-diversity pathogen communities present in the gut of humans during prolonged critical illness.

UnlabelledWe analyzed the 16S rRNA amplicon composition in fecal samples of selected patients during their prolonged stay in an intensive care unit (ICU) and observed the emergence of ultra-low-diversity communities (1 to 4 bacterial taxa) in 30% of the patients. Bacteria associated with the genera Enterococcus and Staphylococcus and the family Enterobacteriaceae comprised the majority of these communities. The composition of cultured species from stool samples correlated to the 16S rRNA analysis and additionally revealed the emergence of Candida albicans and Candida glabrata in ~75% of cases. Four of 14 ICU patients harbored 2-member pathogen communities consisting of one Candida taxon and one bacterial taxon. Bacterial members displayed a high degree of resistance to multiple antibiotics. The virulence potential of the 2-member communities was examined in C. elegans during nutrient deprivation and exposure to opioids in order to mimic local conditions in the gut during critical illness. Under conditions of nutrient deprivation, the bacterial members attenuated the virulence of fungal members, leading to a "commensal lifestyle." However, exposure to opioids led to a breakdown in this commensalism in 2 of the ultra-low-diversity communities. Application of a novel antivirulence agent (phosphate-polyethylene glycol [Pi-PEG]) that creates local phosphate abundance prevented opioid-induced virulence among these pathogen communities, thus rescuing the commensal lifestyle. To conclude, the gut microflora in critically ill patients can consist of ultra-low-diversity communities of multidrug-resistant pathogenic microbes. Local environmental conditions in gut may direct pathogen communities to adapt to either a commensal style or a pathogenic style.ImportanceDuring critical illness, the normal gut microbiota becomes disrupted in response to host physiologic stress and antibiotic treatment. Here we demonstrate that the community structure of the gut microbiota during prolonged critical illness is dramatically changed such that in many cases only two-member pathogen communities remain. Most of these ultra-low-membership communities display low virulence when grouped together (i.e., a commensal lifestyle); individually, however, they can express highly harmful behaviors (i.e., a pathogenic lifestyle). The commensal lifestyle of the whole community can be shifted to a pathogenic one in response to host factors such as opioids that are released during physiologic stress and critical illness. This shift can be prevented by using compounds such as Pi-PEG15-20 that interrupt bacterial virulence expression. Taking the data together, this report characterizes the plasticity seen with respect to the choice between a commensal lifestyle and a pathogenic lifestyle among ultra-low-diversity pathogen communities that predominate in the gut during critical illness and offers novel strategies for prevention of sepsis

An Investigation into Quantifying Micron-G Changes in a Gravitational Field of 1G

This project called for the development of an accelerometer designed to be used in conjunction with gravity shielding experiments. The device had to measure local gravitational changes on the order of a few micro-G's (micron-G) with a spatial resolution greater than one measurement per ten square centimeters. Measurements had to be made at a minimum rate of two per second. Tasks included the design, development and demonstration of a prototype. The deliverable consisted of three copies of this final report. The study resulted in the development of a Transversely Suspended Accelerometer (TSA) which met all of the technical specifications. Different generations of the device were demonstrated to NASA/MSFC personnel as they were developed. The final prototype is available for further demonstration and future use. The study draws attention to the fact that the magnetic fields required to produce gravitational shielding may result in apparent decreases in the weights of suspended objects on the order of those attributed to the effect itself. This observation reinforces the need to quantify the influences of the magnetic field on any measurement device used to study gravitational shielding. This task was accomplished for the TSA

Novel Analysis of Oceanic Surface Water Metagenomes Suggests Importance of Polyphosphate Metabolism in Oligotrophic Environments

Polyphosphate is a ubiquitous linear homopolymer of phosphate residues linked by high-energy bonds similar to those found in ATP. It has been associated with many processes including pathogenicity, DNA uptake and multiple stress responses across all domains. Bacteria have also been shown to use polyphosphate as a way to store phosphate when transferred from phosphate-limited to phosphate-rich media – a process exploited in wastewater treatment and other environmental contaminant remediation. Despite this, there has, to date, been little research into the role of polyphosphate in the survival of marine bacterioplankton in oligotrophic environments. The three main proteins involved in polyphosphate metabolism, Ppk1, Ppk2 and Ppx are multi-domain and have differential inter-domain and inter-gene conservation, making unbiased analysis of relative abundance in metagenomic datasets difficult. This paper describes the development of a novel Isofunctional Homolog Annotation Tool (IHAT) to detect homologs of genes with a broad range of conservation without bias of traditional expect-value cutoffs. IHAT analysis of the Global Ocean Sampling (GOS) dataset revealed that genes associated with polyphosphate metabolism are more abundant in environments where available phosphate is limited, suggesting an important role for polyphosphate metabolism in marine oligotrophs.</p